Poster Session: Biology of Pathogens - Mycology

34-P

Colletotrichm species associated with tree tomato and mango in Colombia.
A. M. Rincon (1), M. Cardenas (1), S. RESTREPO (2), P. Jimenez (3)
(1) Universidad de los Andes, Bogota, Colombia; (2) Univ De Los Andes, Bogota, Colombia; (3) Universidad Militar Nueva Granada, Bogota, Colombia

In a previous study, we characterized almost 100 Colletotrichum isolates collected from tree tomato (tamarillo) and mango in important production areas in Colombia. In that study, the use of two genic regions, GAPDH and ITS, allowed us to assign each isolate to a complex, Gloesporioides, Acutatum or Boninense. However, only 3 species could be identified. We decided to use 3 more genic regions to achieve a better resolution in the identifification. These regions were CHS-1 (chitin synthase I gene), ACT (actin gene) and TUB2 (tubulin 2 gene). Using these six molecular markers, we could assign almost all isolates to species. Our results showed that the most common species isolated from tree tomato was C. tamarilloi. We also confirmed our previous results that a new complex, Boninense, could be recovered from these hosts. From mango we obtained higher diversity and several species could be identified. We also evidenced again the lack of concordance between morphological identifications and molecular classifications.