Fig. 6. Transformation of tobacco to express the Cercospora nicotianae vitamin B6 biosynthesis genes, PDX1 and PDX2. Haploid tobacco was co-transformed with PDX1 and PDX2 on plasmids containing selectable markers for kanamycin and bialaphos resistance, respectively. Transformed plants were selected in vitro for kanamycin resistance and further screened in the greenhouse by spraying with bialaphos. Plants with resistance to both markers were put through a chromosome doubling protocol, resulting in the recovery of diploid plants homozygous for both PDX genes.

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