VIEW ARTICLE

A Comparison of the Chlorosis-Inducing Toxin from Pseudomonas coronafaciens with Wildfire Toxin from Pseudomonas tabaci. S. L. Sinden, Pioneering Research Laboratory, Crops Research Division, ARS, USDA, and Department of Plant Pathology, University of Wisconsin, Madison 53706; R. D. Durbin, Pioneering Research Laboratory, Crops Research Division, ARS, USDA, and Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 60:360-364. Accepted for publication 24 September 1969. DOI: 10.1094/Phyto-60-360.

We have tentatively concluded that the chlorosis-inducing toxins from Pseudomonas tabaci and P. coronafaciens are identical or at least very similar. They behaved identically in a number of chromatographic systems. Acid hydrolysis of either toxin yielded tabtoxinine, threonine, and serine in a 1:1:0.1 molar ratio to the toxin. In 0.4 m NaHCO₃, the toxins are converted stepwise into two alkali inactivation products. Acid hydrolysis of the first product yields the same compounds as do the toxins, whereas tabtoxinine was the only ninhydrin-positive compound found in hydrolysates from the second product. Lactic acid was not detected as a component of the toxins.