Poster: Diseases of Plants: Disease Detection & Diagnosis
475-P
Identification and characterization of Zucchini tigre mosaic virus on creeping cucumber in Japan
K. KOBAYASHI (1), H. Shinohara (2), H. Negishi (2), O. Kim (2) (1) Tokyo University of Agriculture (TUA), Japan; (2) Tokyo University of Agriculture (TUA), Japan
Creeping cucumber (Melothria pendula), a wild cucurbit weed, showing mottle and distortion symptoms was observed in Okinawa main island, Japan in September 2015. Total RNA using TRIzol reagent extracted from collected samples was used for reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers (CIFor and CIRev) for potyviruses (Ha et al., 2008) followed by cloning and sequencing. Obtained 637 nucleotides (nts) sequence of partial CI region from isolate Oki-47 showed 86 % with corresponding sequence from Zucchini tigre mosaic virus (ZTMV; accession no. KC345554) as a highest identity. When the whole coat protein (CP) coding region was considered, the nt and amino acid (aa) sequence identities shared 94 % and 97% with Papaya ringspot virus (PRSV; accession no. AB583208) respectively. The compared PRSV isolate was re-classified as one of ZTMV isolates by Romay et al. (2014). Serological test by western blotting assay revealed that isolate Oki-47 was closely related to PRSV, but not to Papaya leaf distortion mosaic virus (PLDMV). Absence of PRSV, PLDMV, and Zucchini yellow mosaic virus (ZYMV) were also confirmed which are known as major causal potyviruses infecting to cucurbits in Japan. This is the first report of ZTMV incidence in Japan as well as creeping cucumber for its natural host.