Poster: Molecular & Cellular Plant-Microbe Interactions: MPMI
758-P
Reimagining the Cauliflower mosaic virus genome as an interactome between host and virus proteins
J. SCHOELZ (1), R. Nelson (2), S. Leisner (3), C. Angel (4) (1) University of Missouri, U.S.A.; (2) The Samuel Roberts Noble Foundation, U.S.A.; (3) University of Toledo, U.S.A.; (4) Cenicafe, Colombia
The genome of Cauliflower mosaic virus (CaMV) was the first plant-associated organism to be completely sequenced, in 1980. The CaMV nucleotide sequence revealed that its genome was composed of seven substantial open reading frames (ORFs), and six proteins were subsequently matched up with their respective ORFs; the seventh putative protein has never been found in CaMV-infected plants. Since the initial CaMV sequence was finalized, numerous studies have been completed to ascertain the functions of the six CaMV proteins, as well as their subcellular localization. Furthermore, many of the CaMV proteins have been the subject of yeast two-hybrid and co-immunoprecipitation screens to characterize the interactions of host and virus proteins. For example, the P6 protein of CaMV is a multifunctional protein with distinct roles in translation, intracellular movement, elicitation of defenses in resistant hosts or symptoms in susceptible hosts, and modulation of plant defenses. The P6 protein has been shown to interact with at least 14 host and virus proteins. This presentation will illustrate how interactome maps coupled with subcellular localization studies can be used to describe the CaMV disease cycle.