Oral: The Function and Mechanism of CRISPR and Its Applications
70-S
CRISPR/dCas9 technologies for transcriptome manipulation and disease research
S. QI (1) (1) Stanford University, U.S.A.
The bacterial CRISPR/Cas9 system offers a powerful approach for sequence-specific genome engineering in diverse organisms. In addition to genome editing, we have developed the nuclease-dead dCas9 technology and methods for temporal and multi-gene regulation. We recently created a general orthogonal dCas9-based platform to enable parallel transcriptome manipulation. We further applied the technologies to address the needs for genetic disease modeling using human stem cells and the non-editing based approaches for therapeutic applications.