A Virus-Inducible Tobacco Gene Encoding a Glycine-Rich Protein Shares Putative Regulatory Elements with the Ribulose Bisphosphate Carboxylase Small Subunit Gene. Jan A. L. van Kan. Department of Biochemistry, Leiden University, Wassenaarseweg 64, 2333AL Leiden. Ben J. C. Cornelissen(2), and John F. Bol(1). (1) Department of Biochemistry, Leiden University, Wassenaarseweg 64, 2333AL Leiden and (2) Mogen International N. V., Einsteinweg 97, 2333 CB Leiden, The Netherlands.. MPMI 1:107-112. Accepted 15 February 1988. Copyright 1988 The American Phytopathological Society.

cDNA to an mRNA that is strongly induced in Samsun NN tobacco after tobacco mosaic virus (TMV) infection or salicylic acid treatment was used to probe a genomic blot and to screen a genomic library. The mRNA corresponds to a family of approximately eight genes, four of which were cloned. The sequence of the genes and flanking DNA in two clones was determined. One gene was found to contain an intron of 555 bp; S₁-nuclease mapping studies indicated that this gene is expressed. The other gene is interrupted by an intron of 1,954 bp and is probably not expressed after TMV infection. The genes encode a protein of 109 amino acids with a putative N-terminal signal peptide of 26 amino acids. The protein contains a high proportion of glycine (25%) and charged amino acids (29%), suggesting that it may be a cell wall component. A comparison of the upstream sequences of the genes encoding the glycine-rich protein and the pathogenesis-related protein 1a showed only limited homology, although both genes are TMV- and salicylic acid-inducible. However, the upstream sequence of the glycine-rich protein gene contains a 64-bp inverted repeat that occurs in a similar position in the tobacco ribulose bisphosphate carboxylase small subunit gene.