Research Notes Identification of the NodC Protein in the Inner but Not the Outer Membrane of Rhizobium leguminosarum. M. A. Barny. John Innes Institute, John Innes Centre, Colney Lane, Norwich NR4 7UH, U.K.. J. A. Downie. John Innes Institute, John Innes Centre, Colney Lane, Norwich NR4 7UH, U.K.. MPMI 6:669-672. Accepted 24 May 1993. Copyright 1993 The American Phytopathological Society.

An antiserum specific for the NodC protein of Rhizobium leguminosarum reacted with NodC in the inner, but not the outer, membrane fraction. In addition, the NodC protein in intact cells was resistant to proteolytic cleavage. Therefore, contrary to previous observations, it appears that NodC is not exposed on the outer membrane but is localized exclusively in the inner membrane.