An Open Reading Frame Downstream of Rhizobium meliloti nodQ1 Shows Nucleotide Sequence Similarity to an Agrobacterium tumefaciens Insertion Sequence. Julie Schwedock. Department of Biological Sciences, Stanford University Stanford CA 94305-5020 U.S.A. Sharon R. Long. Department of Biological Sciences, Stanford University Stanford CA 94305-5020 U.S.A. MPMI 7:151-153. Accepted 14 September 1993. Copyright 1994 The American Phytopathological Society.

We sequenced a small uncharucterized region in the Rhizobium meliloti nod gene cluster downstream of nodQl. We found the beginning of a large open reading frame (260 amino acids) in this fragment. The sequence reported here has striking similarity to IS66 (Y. Ma-chida, M. Sakurai, S. Kiyokawa, A. Ubasawa, and S. Yasushiro, 1984, Proc. Natl. Acad. Sci. USA 81:7495-7499), an insertion element found in an Agrobacterium tumefaciens mutant.