VIEW ARTICLE

Variation in Embryo Infection and Seed Transmission of Barley Stripe Mosaic Virus Within and Between Two Cultivars of Barley. T. W. Carroll, Department of Botany and Microbiology, Montana State University, Bozeman 59715; S. R. Chapman, Department of Plant and Soil Science, Montana State University, Bozeman 59715. Phytopathology 60:1079-1081. Accepted for publication 17 February 1970. DOI: 10.1094/Phyto-60-1079.

Atlas (C.I. 4118) and Hypana (C.I. 11772) were inoculated with type strain of barley stripe mosaic virus at the three-leaf stage in field studies. At jointing, plants not showing symptoms were rogued. At maturity, 10 random infected plants of each variety were harvested individually, and a composite sample of 100 random heads of each variety was taken. Embryo infection of seed of the individual plants, based on a maximum sample of 50 seeds, and of the composite sample were determined serologically. In the greenhouse, seed transmission was assayed in seedling progeny of the field-grown plants and composite sample as the ratio of seedlings expressing visible symptoms of barley stripe mosaic to total seedlings emerged. Hypana had a lower level of embryo infection and seed transmission than Atlas. Using heterogeneity X², with the level of incidence in the composite samples as the expected value, it was verified that there is significantly greater plant to plant variation in Atlas than in Hypana. The same pattern of variation was detected for both embryo infection and seed transmission.