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Evidence for the Metabolic Detoxification of n-dodecylguanidine Acetate by Ungerminated Macroconidia of Fusarium solani f. sp. phaseoli. J. A. Bartz, Research Assistant, Department of Plant Pathology, University of Wisconsin, Madison 53706, Present address of senior author: Department of Plant Pathology, University of Florida, Gainesville 32601; J. E. Mitchell, Professor, Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 60:350-354. Accepted for publication 22 September 1969. DOI: 10.1094/Phyto-60-350.

Macroconidia of Fusarium solani f. sp. phaseoli sorbed dodine-¹⁴C from aqueous solution and subsequently released a ¹⁴C-labeled material that differed from the parent molecule. When 10 µg of dodine-¹⁴C was supplied to 10⁷ conidia/ml, 63% of the dosage was sorbed in 30 min. After 96 min of exposure, however, two-thirds of this had been altered and released. The labeled compound that was released was much less toxic than dodine, and differed in solubility and chromatographic properties. The process apparently was dependent upon metabolic action, since it did not occur in boiled conidia, and was delayed in cycloheximide-treated spores and in spores held at 0 C. The rate of release of the detoxified material was temperature-dependent. Metabolic detoxification of dodine may be the mechanism by which F. solani f. sp. phaseoli is resistant to the action of dodine.