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Membrane-Bound Ribosomes in Germinated Uredospores. R. C. Staples, Boyce Thompson Institute, Yonkers, New York 10701; L. Ramakrishnan(2), and Z. Yaniv(3). (2)(3)Boyce Thompson Institute, Yonkers, New York 10701. Phytopathology 60:58-62. Accepted for publication 25 July 1969. DOI: 10.1094/Phyto-60-58.

Ribosomes from differentiated uredospores of the bean rust fungus (Uromyces phaseoli) that were germinated for 4 hr were partly bound to cellular membranes. Compared with total ribosomal RNA extracted by a phenol and sodium lauryl sulfate procedure, 37% of the ribosomes were extracted directly, while the remainder were bound to membranes and required release to be recovered. The percentage of ribosomes that were aggregated into polyribosomes was 57%. These polyribosomes were ribonuclease-sensitive. Ribosomes extracted with trypsin were not active in amino-acid incorporation.