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Changes in Susceptible and Resistant Red Clover Epidermal Cells After Infection with Erysiphe polygoni. P. C. Leong, Botany Department, The University of Western Ontario, London, Canada; W. E. McKeen(2), and R. Smith(3). (2)(3)Botany Department, The University of Western Ontario, London, Canada. Phytopathology 60:681-683. Accepted for publication 7 November 1969. DOI: 10.1094/Phyto-60-681.

Susceptible and resistant red clover epidermal cells stain with Azure B immediately after infection with Erysiphe polygoni, whereas normal host cells do not. The susceptible infected cells and, in some instances, the adjoining cells stain light blue, then change to purple after 2 or 3 weeks. The periphery of the infected resistant cells become greenish 1 to 2 days after infection. After 4 to 7 days, the whole cell becomes deep blue. Benedict’s solution turned resistant cells, which had been infected, a yellowish color, while healthy epidermal clover cells remained uncolored. In susceptible infected cells only a faint yellowish color was observed during the 1st week of infection. Resistant infected cells were a yellowish brown 1 or 2 days after infection. These results indicate that specific cells rather than diseased and healthy areas should be compared.