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The State and Infectivity of Tobacco Mosaic Virus in Flue-Cured Tobacco Tissue. G. E. Harman, Research Associate, Department of Plant Pathology, North Carolina State University, Raleigh 27607, Present address of senior author: Assistant Professor, Department of Seed Investigations, New York State Agricultural Experiment Station, Geneva 14456; G. V. Gooding, Jr.(2), and T. T. Hebert(3). (2)(3)Associate Professor, and Professor, respectively, Department of Plant Pathology, North Carolina State University, Raleigh 27607. Phytopathology 61:1032-1033. DOI: 10.1094/Phyto-61-1032.

Tobacco mosaic virus (TMV) from flue-cured tobacco leaf tissue was 7-19% as infectious as TMV from fresh tissue. The ultraviolet absorption spectra of preparations from fresh and flue-cured tissues were similar, and absorption at 265 nm was an accurate measure of the amount of TMV present. Although purified TMV from flue-cured or fresh tobacco reacted similarly in serological tests, the lowest concentration of TMV that exhibited birefrigence was about 4 times higher for TMV from flue-cured tobacco than for TMV from fresh tissue.