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Observation on the Size and Morphology of Cauliflower Mosaic Virus Deoxyribonucleic Acid. R. J. Shepherd, Department of Plant Pathology, University of California, Davis 95616; R. J. Wakeman, Department of Plant Pathology, University of California, Davis 95616. Phytopathology 61:188-193. Accepted for publication 21 September 1970. DOI: 10.1094/Phyto-61-188.

Two different procedures have been used to prepare deoxyribonucleic acid (DNA) from cauliflower mosaic virus for visualization in the electron microscope. With both procedures, DNA filaments exhibited a random orientation when immobilized on positively charged films fixed to electron microscope grids. Two types of molecules, circular and linear, with the same contour length (2.31 ± 0.24 µ) were evident in all preparations examined.

Additional keywords: crucifer viruses, nucleic acids.