VIEW ARTICLE

Tristeza Virus in Citrus reticulata and C. tankan. Ming- hsiung Chen, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan; T. Miyakawa(2), and C. Matsui(3). (2)(3)Horticultural Experiment Station, Katsuura, Tokushima, Japan. Phytopathology 61:279-282. Accepted for publication 22 September 1970. DOI: 10.1094/Phyto-61-279.

Long flexuous particles 2,000 × 12-14 mµ were observed in dip specimens prepared from veinal tissues of Likubin-diseased Citrus reticulata or C. tankan. Electron microscopy of ultrathin sections from diseased plants showed long flexuous particles restricted to the phloem cell. These intracellular filamentous particles were about 10 mµ in width, and most of them appeared as fibrous masses. In some cells, the filamentous particles were scattered randomly throughout the cells. Although the intracellular concentration of these filamentous particles was rather high, the phloem cells containing the filamentous particles were small in number. It was considered that the long flexuous particles observed in the present study corresponded to those described for citrus tristeza virus. It is not clear whether Likubin-diseased plants contain more than one agent or not.