VIEW ARTICLE

Microslide Ouchterlony Technique for Serological Detection of Potato Virus X. R. C. McCrum, Associate Professor, Department of Botany and Plant Pathology, University of Maine, Orono 04473; J. C. Studenroth(2), and Danuta Olszewska(3). (2)(3)University Fellow, and Visiting Scientist, respectively, Department of Botany and Plant Pathology, University of Maine, Orono 04473, (3)Present address: Sopot, ul. Kazimierza, Wielkiego 7m24, Poland. Phytopathology 61:290-292. Accepted for publication 23 September 1970. DOI: 10.1094/Phyto-61-290.

Microslide Ouchterlony methods employing plastic templates enabled the detection and comparison of strains of intact, native, potato virus X. Crude juice-saline (1:1) extracts of potato and Nicotiana glutinosa as well as purified virus from Nicotiana tabacum and Nicotiana glutinosa were used as antigen sources in obtaining positive reactions in gel double-diffusion tests against a commercially available source of antiserum.