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A New Adaptation of Tissue Implantation for the Study of Virus and Mycoplasma Diseases. A. W. Dimock, Department of Plant Pathology, Cornell University, Ithaca, New York 14850; C. M. Geissinger(2), and R. K. Horst(3). (2)(3)Department of Plant Pathology, Cornell University, Ithaca, New York 14850. Phytopathology 61:429-430. DOI: 10.1094/Phyto-61-429.

A newly recognized disease of chrysanthemum, chlorotic mottle, presumably of virus etiology, was efficiently transmitted by inserting cores of tissue removed from stems of diseased plants with a surgical cannula into stems of healthy plants from which similar cores had been removed with a slightly smaller cannula. The technique enabled extremely rapid tissue implantation, and consistently resulted in nearly 100% disease transmission.