VIEW ARTICLE

Incorporation of Thymidine-³H into Carnation Etched Ring Virus. I. Fujisawa, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan; M. Rubio-Huertos(2), and C. Matsui(3). (2)(3)Instituto “Jaime Ferran” de Microbiologia, C. S. I. C., Madrid, Spain. Phytopathology 61:681-684. Accepted for publication 21 January 1971. DOI: 10.1094/Phyto-61-681.

Characteristic X-bodies occurred in leaves of Dianthus spp. infected systemically with carnation etched ring virus. Most X-bodies were round and smaller than the nuclei. The X-bodies consisted of an amorphous matrix, vacuolelike spaces, and spherical particles about 40-45 nanometers in diameter. These general profiles of X-bodies associated with carnation etched ring virus infection resembled those of viruses belonging to the cauliflower mosaic virus group. According to purification and sucrose density-gradient centrifugation of extract from infected leaves labeled with thymidine-³H, the radioactivity was in one fraction. Since this fraction consisted of spherical particles about 47 nanometers in diameter, it was concluded that carnation etched ring virus contained deoxyribonucleic acid. Carnation etched ring virus is a possible member of the cauliflower mosaic virus group.