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Bearded Iris Mosaic Virus: Transmission, Purification, Inclusions, and its Differentiation from Bulbous Iris Mosaic. O. W. Barnett, Assistant Professor, Department of Plant Pathology and Physiology, Clemson University, Clemson, South Carolina 29631; G. A. de Zoeten(2), and G. Gaard(3). (2)(3)Associate Professor and Electron Microscopist, respectively. Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 61:926-932. Accepted for publication 3 March 1971. DOI: 10.1094/Phyto-61-926.

A virus from bearded iris in Wisconsin, Scotland, and South Carolina was easily transmitted to Belamcanda chinensis. The virus had a restricted host range, and was aphid-transmitted in a nonpersistent manner. In B. chinensis, sap infectivity was lost after 36 hours of aging, heating at 50 C for 10 min, or when diluted 1 × 10^–4. Purified particles had a modal length of 753 nm, and inclusions in B. chinensis were similar to those observed in plants infected with viruses from the potato virus-Y group. Antiserum conjugated to latex was used to show relations of the purified R58 isolate with several isolates from bearded iris in Scotland and South Carolina. The bearded iris virus was not serologically related to a virus causing mild mosaic in bulbous iris, and the host ranges of the two viruses differed.