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Plant and Aphid Protein Patterns as Influenced by Pea Enation Mosaic Virus. G. A. de Zoeten, Associate Professor, Department of Plant Pathology, University of Wisconsin, Madison 53706; Nira Rettig, Research Associate, Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 62:1018-1023. Accepted for publication 20 March 1972. DOI: 10.1094/Phyto-62-1018.

Isoelectric focusing was used to study changes in protein patterns and some enzyme activities in pea plants (Pisum sativum ‘Perfected Wales’) infected with pea enation mosaic virus (PEMV) and in viruliferous and non-viruliferous aphids (Myzus persicae and Acyrthosiphon pisum). A peroxidase-active band with an isoelectric point at pH 7.2, which did not result from accelerated senescence, was observed in patterns developed from protein extracts of diseased peas, but not in patterns of noninfected pea tissue. Viruliferous pea aphids (Acyrthosiphon pisum) contained a protein with an isoelectric point at pH 5.1 which was not present in extracts from nonviruliferous aphids and did not react with PEMV antiserum. A similar effect of the virus on Myzus persicae, green peach aphid, was not found.