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Metabolic Behavior of Pseudomonas savastanoi Isolates from Olive and Oleander on Certain Carbohydrate and Amino Substrates. E. E. Wilson, Professor Emeritus, Department of Plant Pathology, University of California, Davis 95616; M. G. Heskett(2), Marilyn L.(3), Johnson(4), and T. Kosuge(5). (2)(3)(4)(5)Laboratory Technicians, and Professor, respectively, Department of Plant Pathology, University of California, Davis 95616. Phytopathology 62:349-355. Accepted for publication 22 October 1971. DOI: 10.1094/Phyto-62-349.

Pseudomonas savastanoi from tumors on olive and oleander did not differ significantly in oxidative metabolic patterns on twelve substrates. When cells were cultured in a medium with D-glucose as the carbon source, oxygen absorption in respirometer experiments was the highest on alanine, followed in descending order by fructose, serine, glucose, glycerol, glutamine, asparagine, and galactose. Oxygen uptake was scant or zero on sucrose, xylose, phenylalanine, and tryptophan. On a complete medium containing sucrose, however, most oleander isolates grew and produced measurable amounts of acid. Such isolates grown in a sucrose medium readily oxidized sucrose in respirometer tests. An inducible sucrose transport system and an inducible beta-fructofuranosidase (beta-D-fructo-furanoside fructohydrolase, E.C. No. 3.2.1.26) allows utilization of sucrose by these isolates. With one exception, olive isolates that were tested apparently lack the capacity to produce both systems.