VIEW ARTICLE

Electron Microscopy of Intracellular Radish Mosaic Virus. Y. Honda, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan; C. Matsui, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan. Phytopathology 62:448-452. Accepted for publication 15 November 1971. DOI: 10.1094/Phyto-62-448.

Masses of radish mosaic virus particles occurred on the interface between the cytoplasm and vacuole or in the vacuole. In these masses, some virus particles aligned in crystal rows. Presumably, the virus was formed within the cytoplasm and liberated into the vacuole. The most prominent modification was invagination of the tonoplast into the cytoplasm. The virus particles attached to the tonoplast in the vacuole were incorporated into the tonoplastic channel during invagination of the tonoplast into the cytoplasm. Within the tonoplastic channels, the virus particles were aligned in multi-rows. The virus particles were seen in the plasmodesmata across the cell wall. Because the above-mentioned profiles of the virus particles were rather scarce, it is likely that most virus particles scatter randomly throughout the cytoplasm.