VIEW ARTICLE

Production of the Phytoalexin, Hydroxyphaseollin, in Soybean Leaves Inoculated with Tobacco Necrosis Virus. W. L. Klarman, Associate Professor, Department of Botany, University of Maryland, College Park, Maryland 20742; F. Hammerschlag, Former Graduate Assistant, Department of Botany, University of Maryland, College Park, Maryland 20742. Phytopathology 62:719-721. Accepted for publication 31 January 1972. DOI: 10.1094/Phyto-62-719.

The phytoalexin, hydroxyphaseollin (HP), was detected in soybean leaves 30 hr after inoculation with tobacco necrosis virus (TNV). Maximal concentration occurred 48 to 72 hr after inoculation, but HP decreased to approximately two-thirds of the maximum after 96 hr. Quantity of HP was proportional to the number of lesions per leaf provided sufficient uninfected tissue separated the lesions. When lesion number was high, HP concentration decreased. Hydroxyphaseollin was not detected in the noninoculated tissue, although it was noted that inoculation of one primary leaf with TNV interferred with the establishment of TNV lesions in the opposite leaf when inoculated 48 hr later.