VIEW ARTICLE

Infection of Isolated Plant Protoplasts with Potato Virus X. T. A. Shalla, Department of Plant Pathology, University of California, Davis 95616; L. J. Petersen, Department of Plant Pathology, University of California, Davis 95616. Phytopathology 63:1125-1130. Accepted for publication 16 March 1973. DOI: 10.1094/Phyto-63-1125.

Tobacco leaf protoplasts were inoculated in vitro with potato virus X (PVX) and monitored for newly formed infection products by fluorescent antibody staining, electron microscopy, and infectivity measurements. Viral antigen, PVX particles, and laminate inclusion body components (beaded sheets) in the cytoplasm were observed at 38, 56, and 62 hr, respectively, after inoculation. Intracytoplasmic particles were identified as PVX by ferritin-antibody labeling. Microscopic indications of infection were associated with a sixfold increase of infectious material.