VIEW ARTICLE

Extraction of the Curly Top Agent from Segmented Petioles of Beet. K. Izadpanah, Department of Plant Pathology, University of California, Davis 95616, Senior author on leave from Pahlavi University, Shiraz, Iran; R. J. Shepherd, Department of Plant Pathology, University of California, Davis 95616. Phytopathology 63:1209-1210. DOI: 10.1094/Phyto-63-1209.

A convenient and effective technique for extracting the curly top causal agent consists of immersing 4- to 6-mm segments of recently infected beet petiole and leaf midrib for 24 to 48 hr in 0.01 M sodium citrate containing 0.1 to 0.4% 2-mercaptoethanol. Beet leafhoppers fed upon such extracts transmitted at rates comparable to those reported for leafhoppers fed on conventional phloem exudates from beets.