VIEW ARTICLE

Antiserum Preparation and Serodiagnosis of Pea Seed-Borne Mosaic Virus. W. R. Stevenson, Department of Plant Pathology, University of Wisconsin, Madison 53706; D. J. Hagedorn, Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 63:1467-1469. Accepted for publication 15 May 1973. DOI: 10.1094/Phyto-63-1467.

Two antisera, one using intact pea seed-borne mosaic virus (PSbMV) particles as the antigen, and one using D-protein from PSbMV particles, were prepared. These antisera, when used in the microprecipitin test, could be used to identify PSbMV in partially purified virus extracts. The identification of PSbMV in clarified sap from naturally infected pea plants was possible using the antiserum to intact PSbMV in the microprecipitin procedure. Attempts to utilize agar double-diffusion tests with either antiserum were unsuccessful.