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Production of Ammonia by Tobacco and Soybean Inoculated with Bacteria. Clyde Hurst, Research Specialist, Department of Plant Pathology, University of Minnesota, St. Paul 55101, Present address: Ecological Services Laboratory, National Park Service, Washington, D.C. 20242; B. W. Kennedy(2), and L. Olson(3). (2)Professor, Department of Plant Pathology, University of Minnesota, St. Paul 55101; (3)Assistant Professor, Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Present address: Christopher Newport College, College of William and Mary, Newport News, Virginia 23601. Phytopathology 63:241-242. Accepted for publication 28 September 1972. DOI: 10.1094/Phyto-63-241.

Ammonia was consistently produced by tobacco leaves inoculated with Pseudomonas tabaci, but not by tobacco leaves inoculated with P. pisi, even though the symptoms caused by the two species of bacteria were indistinguishable. There was no correlation between susceptible, resistant, or hypersensitive reaction and evolution of ammonia by tobacco or soybeans. Soybean leaves and callus tissue inoculated with bacteria did not produce more ammonia than did the controls.

Additional keywords: hypersensitivity, Glycine max, Nicotinia tabacum.