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Use of Mycelial Suspension for Increasing Puccinia graminis f. sp. tritici in Axenic Culture. H. W. Mussell, Assistant Plant Pathologist, Boyce Thompson Institute for Plant Research, 1086 North Broadway, Yonkers, N.Y. 10701; R. C. Staples, Biochemist, Boyce Thompson Institute for Plant Research, 1086 North Broadway, Yonkers, N.Y. 10701. Phytopathology 63:653-654. DOI: 10.1094/Phyto-63-653.

Axenic cultures of Puccinia graminis f. sp. tritici race 126-ANZ-6,7 were initiated using suspensions of mycelium prepared by blending stroma of the fungus in a liquid culture medium. Over a 5-month period, cultures derived from one 50-mg section of fungal stroma yielded 1,325 vigorously growing colonies. The fungus was capable of infecting wheat plants after three successive subcultures using mycelial suspensions.