VIEW ARTICLE

Polyethylene Glycol for Purification of Potato Yellow Dwarf Virus. H. T. Hsu, Research Associate, Department of Botany, University of Illinois, Urbana 61801; L. M. Black, Professor, Department of Botany, University of Illinois, Urbana 61801. Phytopathology 63:692-696. Accepted for publication 14 December 1972. DOI: 10.1094/Phyto-63-692.

Polyethylene glycol of 6,000 molecular weight (PEG 6,000) was used to improve the purification of potato yellow dwarf virus. Optimal conditions of precipitation were determined to retain maximal amounts of the initial infectivity in the final preparation. Separation of green host pigment from virus was better in PEG-treated preparations than in controls in which virus was concentrated by differential centrifugation. PEG concentrations of 7 and 4% were optimal for the sanguinolenta and constricta varieties of the virus, respectively, but 0.1 M NaCl and pH 7.0 were optimal for both.