VIEW ARTICLE

New Medium and Conditions for the Culture of Monolayers of Agallian Leafhopper Cells Used in Studies of Certain Plant Viruses. G. Martinez -Lopez, Research Associate, Provisional Department of Genetics and Development, University of Illinois, Urbana 61801, Present address of senior author: Department of Plant Pathology, Instituto Colombiano Agropecuario, Apartado Aereo 151123 (E1 Dorado), Bogota, D.E. Colombia, S.A; L. M. Black, Professor, Provisional Department of Genetics and Development, University of Illinois, Urbana 61801. Phytopathology 64:1040-1041. DOI: 10.1094/Phyto-64-1040.

Established cell monolayers of Agallia constricta, an insect vector of certain plant viruses, were used to determine what concentrations of the medium components used by Chiu and Black were optimal for growth. Optimal pH and osmotic pressure were also determined. As a result, a new medium was prepared in which the cells grew more rapidly, were more susceptible to inoculation, and yielded more virus.