VIEW ARTICLE

Extraction of Fluid from Healthy and Dutch-Elm-Diseased Elm Branches using Hydraulic Compression. Alice C. Woods, Research Assistant, Department of Plant Pathology, Agricultural Experiment Station, College of Food and Natural Resources, University of Massachusetts, Amherst, Mass. 01002, Present address of senior author: 901 Dryden Rd., Ithaca, New York 14850; Francis W. Holmes, Professor and Director of Shade Tree Laboratories, Department of Plant Pathology, Agricultural Experiment Station, College of Food and Natural Resources, University of Massachusetts, Amherst, Mass. 01002. Phytopathology 64:1265-1267. DOI: 10.1094/Phyto-64-1265.

Extracts were obtained from Dutch-elm-diseased and healthy elm branches using hydraulic compression. Phenol oxidation was inhibited by using low temp, N₂ atmospheres, and sodium metabisulphite during extraction. Polygalacturonase and phosphatidase activity were detected in both diseased and healthy tissue extracts, polygalacturonase being greater in the diseased tissue extracts.