VIEW ARTICLE

Purification of Curly Top Virus. G. I. Mink, Plant Pathologist, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350; P. E. Thomas, Research Plant Pathologist, USDA, ARS, Western Region, Prosser, Washington. Phytopathology 64:140-142. Accepted for publication 9 July 1973. DOI: 10.1094/Phyto-64-140.

Curly top virus was partially purified from charcoal-clarified homogenates of frozen bean (phaseolus vulgaris L. ‘Bountiful’) stem tissue by differential and density-gradient ultracentrifugation. In rate sucrose density-gradient tubes, infectivity was associated with a visible band located 14-17 mm below the tube meniscus. The infectious band contained isometric particles having about 20% nucleic acid content and a sedimentation coefficient of 82 S. Traces of contaminating host materials were detected by cellulose acetate strip electrophoresis.