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Improved Methods for the Axenic Culture of Puccinia recondita f. sp. tritici. S. A. Raymundo, Instructor, Department of Botany and Plant Pathology, Oklahoma State University, Stillwater 74074; H. C. Young, Jr., Professor, Department of Botany and Plant Pathology, Oklahoma State University, Stillwater 74074. Phytopathology 64:262-263. DOI: 10.1094/Phyto-64-262.

Improved methods for obtaining axenic growth of Puccinia recondita f. sp. tritici are described. Details of techniques for production of sterile uredospore inoculum, seeding of culture medium, and maintenance and care of established cultures, are presented. The improved methods consistently yielded 75 to 90% successful seedings in each trial, compared to erratic results yielding 0 to 40% obtained with previous methods.