VIEW ARTICLE

Ultrastructure of Tobacco Mesophyll Protoplasts Inoculated with Cucumber Mosaic Virus. Y. Honda, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan; C. Matsui(2), Y. Otsuki(3), and I. Takebe(4). (2)Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan; (3)(4)Institute for Plant Virus Research, 959 Aobacho, Chiba, Japan. Phytopathology 64:30-34. Accepted for publication 15 June 1973. DOI: 10.1094/Phyto-64-30.

Mesophyll protoplasts isolated from tobacco plants were inoculated in vitro with cucumber mosaic virus and were examined by thin sectioning at intervals after inoculation. Inoculum virus particles entered the protoplasts by a pinocytic process. Progeny virus particles were readily detectable 24 hr after inoculation as aggregates and were associated with the plasmalemma, tonoplast, and nucleolus, or were present in the cytoplasm. No virus particles were found in chloroplasts, mitochondria, or central vacuole. In the nuclei containing CMV particles, heterochromatin was not seen and the nucleolus was sometimes vacuolated. No other degenerative changes were found in the protoplasts until 48 hr after inoculation.