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Deoxyribonucleic Acid in Dahlia Mosaic Virus. I. Fujisawa, M. Rubio-Huertos, and C. Matsui; Faculty of Agriculture, Nagoya University, Nagoya, Japan; Instituto Jaime Ferran de Microbiologia, CSIC, Madrid, Spain. Phytopathology 64:287-290.

Dahlia mosaic virus was purified from infected Verbesina encelioides by differential centrifugation and sucrose density-gradient centrifugation.  The purified preparations revealed a typical ultraviolet absorption spectrum for a nucleoprotein, and consisted of infective particles about 45 nm in diam.  Thymidine-³H was incorporated preferentially into the virus and the nucleic acid isolated from the purified virus was digested completely with deoxyribonuclease, but not digested with ribonuclease.  The virus therefore contains deoxyribonucleic acid.  X-bodies associated with dahlia mosaic virus infection were stained with conjugated anti-cauliflower mosaic virus globulin.