VIEW ARTICLE

Chromatographic Estimation of Fungal Mass in Plant Materials. Lung- Chi Wu, Visiting Professor, Department of Plant Pathology, and Research Associate, Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison 53706; M. A. Stahmann, Professor, Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison 53706. Phytopathology 65:1032-1034. DOI: 10.1094/Phyto-65-1032.

The determination of glucosamine from fungal chitin following acid hydrolysis, ion-exchange chromatography, and reaction with ninhydrin, provides a simple rapid method to estimate the quantity of fungi in plant tissue and fungal cultures. The use of chromatography and ninhydrin circumvents some disadvantages of other colorimetric methods and allows use of the automatic amino acid analyzer.