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A Qualitative Method for Detecting Xanthomonas campestris in Crucifer Seed. N. W. Schaad, Assistant Professor, Department of Plant Pathology, University of Georgia College of Agriculture Experiment Stations, Georgia Station, Experiment 30212; Robyn Kendrick, High School DCT Lab Helper, Department of Plant Pathology, University of Georgia College of Agriculture Experiment Stations, Georgia Station, Experiment 30212. Phytopathology 65:1034-1036. DOI: 10.1094/Phyto-65-1034.

An assay procedure using water-enriched cabbage seeds on plating media containing cycloheximide was developed for detecting low numbers of Xanthomonas campestris, the causal agent of black rot of crucifers. Using inoculated seed, this method was capable of detecting a single diseased seed per 1,000 and was superior to several other methods tested. A commercial lot of cauliflower seed was shown to contain 0.8% diseased seed. A suggested assay procedure is described.