VIEW ARTICLE

A Medium for Enumeration and Isolation of Calonectria crotalariae from Soil. S. C. Hwang, Graduate Research Assistant, Department of Plant Pathology, University of Hawaii, Beaumont Agricultural Research Center, Hilo 96720; W. H. Ko, Associate Professor, Department of Plant Pathology, University of Hawaii, Beaumont Agricultural Research Center, Hilo 96720. Phytopathology 65:1036-1037. DOI: 10.1094/Phyto-65-1036.

Calonectria crotalariae produced brownish colonies with visible strands of microsclerotia on acidified medium containing 2% dextrose, 0.02% peptone, and 2% agar. The colony size and growth of aerial mycelia of soil fungi were greatly reduced on this medium due to the high dextrose-peptone ratio. With this medium 83 to 93% of C. crotalariae propagules were recovered from artificially inoculated soil. The population of this fungus in the vicinity of diseased papaya seedlings ranged from 350 to 3,050 propagules per gram of dried soil.