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Taxonomic Relationships Among Typhula Species as Revealed by Mating Experiments. G. W. Bruehl, Department of Plant Pathology, Washington State University, Pullman 99163; R. Machtmes(2), and R. Kiyomoto(3). (2)(3)Department of Plant Pathology, Washington State University, Pullman 99163. Phytopathology 65:1108-1114. Accepted for publication 28 April 1975. DOI: 10.1094/Phyto-65-1108.

Typhula idahoensis, T. incarnata, T. ishikariensis, T. trifolii, and an unknown Typhula sp. all had tetrapolar incompatibility, but no distinction could be made between A and B incompatibility factors. Partial sterility was common within natural dikaryons of T. idahoensis, occasional within T. ishikariensis, and rare in T. incarnata. Monokaryons did not give rise to fertile basidiocarps. Interspecific pairings of monokaryons of T. incarnata, T. trifolii, and an unknown Typhula species were incompatible with each other and with T. idahoensis and T. ishikariensis. The latter two species mated to a limited extent, but most of the hybrid offspring were presumed incapable of survival in nature. Mating experiments support continued recognition of these species. Typhula monokaryons are dikaryotized by donor dikaryons of the same species. Pairing dikaryons with monokaryons (di-mon matings) is thus useful in identifying Typhula isolates. Our procedure for using di-mon matings in identifying T. idahoensis, T. incarnata, and T. ishikariensis is given.