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Identification of Tobacco Ringspot Virus in Clerodendrum thomsoniae. Mushtaq A. Khan, Department of Plant Pathology, University of Wisconsin, Madison 53706; D. P. Maxwell, Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 65:1150-1153. Accepted for publication 28 May 1975. DOI: 10.1094/Phyto-65-1150.

The causal agent of ringspot symptoms on Clerodendrum thomsoniae ‘Glorybower’ was mechanically transmitted to Gomphrena globosa. It was purified from n-butanol: chloroform-clarified sap of inoculated Nicotiana tabacum ‘Xanthi-nc’ by differential centrifugation and also by a polyethylene glycol (MW 6,000)-NaCl precipitation procedure. The causal agent was identified as an isolate of tobacco ringspot virus because of its host range, physical properties, particle morphology, and serological relationships. This is the first identification of a virus disease of Clerodendrum spp.