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Procedures for Crystallization and Further Purification of Tentoxin. Suzan H. Woodhead, Research Assistant, Department of Plant Pathology and Department of Chemistry, University of Arkansas, Fayetteville 72701; G. E. Templeton(2), W. L. Meyer(3), and R. B. Lewis(4). (2)(3)(4)Professor of Plant Pathology, Professor of Chemistry, and Postdoctoral Research Associate respectively, Department of Plant Pathology and Department of Chemistry, University of Arkansas, Fayetteville 72701. Phytopathology 65:495-496. DOI: 10.1094/Phyto-65-495.

Procedures for collecting, screening, and maintaining strains of Alternaria tenuis that produce high yields of tentoxin were refined. Tentoxin was crystallized as the benzene solvate after extraction from concentrated filtrates and column chromatography over silicic acid with chloroform:acetone then further purified by removal of the benzene of solvation. Yields ranged from 25-35 mg pure peptide per liter of original filtrate.