VIEW ARTICLE

Technique for Scanning Electron Microscopy of Fungal Structures within Plant Cells. D. A. Kinden, Former Graduate Research Assistant, Department of Plant Pathology, University of Missouri, Columbia 65201, Present address of senior author: Department of Veterinary Pathology, University of Missouri, Columbia 65201; M. F. Brown, Associate Professor, Department of Plant Pathology, University of Missouri, Columbia 65201. Phytopathology 65:74-76. Accepted for publication 30 July 1974. DOI: 10.1094/Phyto-65-74.

A procedure for the removal of host cytoplasm from infected cells and the preservation of fungal structures for scanning electron microscopy (SEM) is described. The technique permits the use of SEM to study the morphology and spatial relationships of intracellular structures produced by parasitic fungi. Haustoria of pathogenic fungi and arbuscules of a vesicular-arbuscular mycorrhizal fungus are illustrated.