VIEW ARTICLE

Vector Relations

Isolation of Cryptoporus volvatus and Fomes pinicola from Dendroctonus pseudotsugae. John D. Castello, Formerly Research Assistant, Department of Plant Pathology, Washington State University, Pullman, WA 99163, Present address of senior author: Department of Plant Pathology, University of Wisconsin, Madison, WI 53706; Charles Gardner Shaw(2), and M. M. Furniss(3). (2)Professor and Plant Pathologist, Department of Plant Pathology, Washington State University, Pullman, WA 99163; (3)Principal Entomologist, Forestry Sciences Laboratory, Intermountain Forest and Range Experiment Station, U.S. Forest Service, Moscow, ID 83843. Phytopathology 66:1431-1434. Accepted for publication 9 June 1976. Copyright © 1976 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-66-1431.

Cryptoporus volvatus and Fomes pinicola were isolated from Douglas-fir beetles (Dendroctonus pseudotsugae) trapped in flight (24% and 37%, respectively). Dendroctonus pseudotsugae is not an inhabitant of sporophores; consequently, mycelial fragments are considered to be the propagules disseminated. Fomes pinicola also was isolated from beetles removed from galleries in Pseudotsuga menziesii. Upon entering the tree after flight, the beetles probably dislodge mycelial fragments during their tunneling activities. Difco malt agar (4.5%) containing benomyl at 30 µg/ml, streptomycin-sulfate at 100 µg/ml, and 85% lactic acid at 4,000 µg/ml provided a selective medium for isolation of C. volvatus and F. pinicola. Results indicate that D. pseudotsugae is a major vector of C. volvatus on P. menziesii in northern Idaho, and also that it is an effective vector of F. pinicola.