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Vector Relations

Serological Properties of Aphid-Transmissible and Aphid-Nontransmissible Pea Enation Mosaic Virus Isolates. R. G. Clarke, Graduate Research Assistant, Department of Entomology, Michigan State University, East Lansing, MI 48824, Senior author Present address: Assistant Professor, Department of Entomology, Oregon State University, Corvallis, OR 97331; James E. Bath, Professor, Department of Entomology, Michigan State University, East Lansing, MI 48824. Phytopathology 67:1035-1040. Accepted for publication 7 February 1977. Copyright © 1977 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-67-1035.

An aphid-transmissible isolate of pea enation mosaic virus (T-PEMV) was compared biologically and serologically with an aphid-nontransmissible isolate (NT-PEMV) of the same virus. Although both isolates were easily transmitted by sap inoculations, NT-PEMV was not transmitted by the pea aphid, despite attempts using three different acquisition methods. Isolate T-PEMV was efficiently transmitted by the pea aphid by all three acquisition methods. Antisera produced in rabbits contained a single antibody population against NT-PEMV (cross-reactive with T-PEMV) but two antibody populations were produced against T-PEMV (one cross-reactive with NT-PEMV and one specific for T-PEMV). A soluble protein concentrated from the high-speed centrifugation supernatant fraction obtained during purification of T-PEMV reacted specifically with T-PEMV antiserum and not with NT-PEMV antiserum. No reactive soluble protein was detected in similar extractions from NT-PEMV infected plants.