VIEW ARTICLE

Etiology

Purification, Serology, and Some Physical Properties of Dasheen Mosaic Virus. M. M. Abo El-Nil, Postdoctoral Fellow, Plant Pathology Department, University of Florida, Gainesville, FL 32611; F. W. Zettler(2), and E. Hiebert(3). (2)(3)Professor, and Associate Professor, respectively, Plant Pathology Department, University of Florida, Gainesville, FL 32611. Phytopathology 67:1445-1450. Accepted for publication 15 June 1977. Copyright © 1977 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-67-1445.

A technique for purifying dasheen mosaic virus (DMV) is described. Antisera prepared to two DMV isolates were virus specific in immunodiffusion tests, and homologous titers of 1:512 were recorded for both isolates in microprecipitin tests. Dasheen mosaic virus is related serologically to blackeye cowpea mosaic and tobacco etch viruses, but not to potato Y or turnip mosaic viruses. The average uncorrected A_260/247 and A_260/280 ratio of each DM isolate was 1.08 ± 0.2 and 1.19 ± 0.2, respectively, and the A₂₆₀ (1 mg/ml, 1-cm light path) was 2.38 ± 0.05. Polyacrylamide gel electrophoresis in 0.1% sodium dodecyl sulfate revealed coat protein heterogeneity and anomalous electrophoretic behavior. Coat protein molecular weights ranged from 25,000–45,000 in 11% polyacrylamide, 28,000-51,000 in 6% polyacrylamide, and 30,000-80,000 in 3% polyacrylamide.