VIEW ARTICLE

Physiology and Biochemistry

The Influence of Bromegrass Mosaic Virus on the Replication of Tobacco Mosaic Virus in Hordeum vulgare. R. I. Hamilton, Research Scientist, Agriculture Canada, Research Station, 6660 N.W. Marine Drive, Vancouver, B.C., Canada V6T 1X2; C. Nichols, technician, Agriculture Canada, Research Station, 6660 N.W. Marine Drive, Vancouver, B.C., Canada V6T 1X2. Phytopathology 67:484-489. Accepted for publication 4 October 1976. Copyright © 1977 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-67-484.

Inoculation of the first leaf of barley (Hordeum vulgare ‘Black Hulless’) with tobacco mosaic virus (TMV) and bromegrass mosaic virus (BMV) resulted in systemic infection of the host by both viruses whereas in plants inoculated with TMV only, the virus remained in the inoculated leaf. The yield of TMV from systemic infection of leaves 2-4 was 5-10 times that in the inoculated leaf. Systemic infection by TMV was dependent upon high temperature (30 C), but a temperature-shift experiment indicated that replication of TMV in systemically infected leaves occurred at 25 C. No evidence was obtained for encapsidation of TMV-RNA in BMV protein. No systemic infection of Black Hulless barley was obtained by inoculation with alfalfa mosaic, cowpea chlorotic mottle, southern bean mosaic, tobacco necrosis, or turnip yellow mosaic viruses in the presence or absence of BMV.