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Detection of Wheat Streak Mosaic Virus Antigens in Vector Mites with Fluorescent Antibodies. R. C. Sinha, Chemistry and Biology Research Institute, Agriculture Canada, Ottawa, Ontario, Canada; Y. C. Paliwal, Chemistry and Biology Research Institute, Agriculture Canada, Ottawa, Ontario, Canada. Phytopathology 67:570-572. Accepted for publication 15 January 1976. DOI: 10.1094/Phyto-67-570.

A fluorescent antibody technique was used to detect wheat streak mosaic virus antigens in crude “homogenates” prepared from 10 mites (Aceria tulipae) that had been exposed to virus-infected plants. The virus antigens also were detected in the body fluid released through the anus of individual exposed mites when pressed gently with a fine glass rod.