VIEW ARTICLE

Cytology and Histology

In Situ Reaction of Clover Yellow Mosaic Virus (CYMV) Inclusion Bodies with Fluorescent Antibodies to CYMV. D. V. Rao, Former Graduate Research Assistant, Department of Plant Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2E3; P. Shukla(2), and C. Hiruki(3). (2)(3)Former Postdoctoral Fellow, and Professor, respectively, Department of Plant Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2E3; (2)Present address: Division of Plant Pathology, C. S. Azad University of Agriculture and Technology, Kanpur-2, India. Phytopathology 68:1156-1159. Accepted for publication 5 January 1978. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-1156.

Amorphous inclusions were observed as diffuse masses of yellowish-green fluorescence in clover yellow mosaic virus (CYMV)-infected cells of the primary leaves of cowpea (Vigna sinensis ‘Black Eye’). The CYMV inclusions identified by immunofluorescence microscopy corresponded to those found by light microscopy and to virus aggregates found by electron microscopy. The immunofluorescence technique should be useful in the identification of virus inclusions in situ.