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Infection and Synthesis Rate of Southern Bean Mosaic Virus in Soybean Callus Cells under Selected Cultural Conditions. Fang- Sheng Wu, Graduate Student, Department of Botany and Plant Pathology, Michigan State University, East Lansing, MI 48824; Harry H. Murakishi, professor, Department of Botany and Plant Pathology, Michigan State University, East Lansing, MI 48824. Phytopathology 68:1389-1392. Accepted for publication 14 March 1978. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-1389.

Soybean (Glycine max ‘Harosoy 63’) callus cells grown in either Eriksson’s or Linsmaier and Skoog’s media were inoculated with southern bean mosaic virus (SBMV) and then incubated in either liquid or agar media. The composition and type (liquid or agar) of the medium significantly affected the growth curve of the virus. Assays in Phaseolus vulgaris ‘Pinto’ leaves indicated that soybean callus grown in liquid and incubated in liquid medium after inoculation gave the most rapid virus growth rate compared with that of Prince bean callus treated identically or with infected soybean plants grown in the greenhouse. Vortexing of the cell-suspension/virus-inoculum mixture as is done for tobacco mosaic virus—tobacco callus infection was not necessary with the soybean callus-SBMV system. Infection was achieved by adding virus inoculum to a suspension culture of callus cells, washing the cells with fresh medium and incubating the cells in liquid media on a rotary shaker at 120 rpm.